MOLECULAR BIOLOGY

Smiley face1.3. PCR TECHNIQUES

We present a series of kits for carrying out various practical applications of the PCR technique, each kit consists of a theoretical part that the teacher work with students and a practical part consisting in PCR and results.

The kits include reagents for conducting electrophoresis and staining (DANABLUE-FlashBlue or GELSAFE, if it is available a transilluminator).

You must have a thermal cycler to carry out these practices.

We can supply the EDVOTEK’s thermal cycler, firm that we represent.

STUDY OF VNTR HUMAN POLYMORPHISMS BY PCR

The DNA sequences that vary between individuals are known as polymorphisms. Students will prepare their own DNA from their mouth cells to be used for PCR and study the DS180 locus VNTR polymorphism.

> Kit includes:
MIX PCR, positive control, agarose, TAE and DANABLUE-FlashBlue or GELSAFE (if it is available a transilluminator). 
> You need:
Micropipettes, PCR, microtubes, and thermal cycler.

icon-refPCR1           25 students

DETERMINING Rh FACTOR BY PCR

Students will prepare their own DNA from their buccal cells to be used for PCR. In a second session, the amplified DNA is analyzed by electrophoresis and it is determinated the Rh factor.

> Kit includes:
MIX PCR, positive control, agarose, TAE and DANABLUE-FlashBlue or GELSAFE (if it is available a transilluminator).
> You need:
Micropipettes, PCR, microtubes, and thermal cycler. 

icon-refPCR2           25 students

STUDY OF ALU HUMAN POLYMORPHISMS BY PCR

The DNA sequences that vary between individuals are known as polymorphisms.. Students will prepare their own DNA from their buccal cells to be used for PCR and study the presence or absence of Alu locus in gene activator of plasmimogen.

> Kit includes:
MIX PCR, positive control, agarose, TAE and DANABLUE-FlashBlue or GELSAFE (if it is available a transilluminator). 
> You need:
Micropipettes, PCR, microtubes, and thermal cycler.

icon-refPCR3           25 students

PCR FROM GEN 16S rRNA OF BACTERIAL

This kit includes all material you need for the amplification of a fragment of the gene 16S rRNA of bacterial using the PCR technique. It is not necessary to isolate bacterial DNA, the kit is provided with a sample of bacterial DNA to perform the amplifications.

> Kit includes:
MIX PCR, positive control, agarose, TAE and DANABLUE-FlashBlue or GELSAFE (if it is available a transilluminator). 
> You need:
Micropipettes, PCR, microtubes, and thermal cycler.

icon-refPCR4           25 students

PCR FROM GEN 18S rRNA OF HUMAN

This kit includes all material you need for the amplification of a fragment of the gene 18S rRNA of human using the PCR technique. It is not necessary the students isolate their DNA, the kit is provided with a sample of human DNA to perform the amplifications.

> Kit includes:
MIX PCR, positive control, agarose, TAE and DANABLUE-FlashBlue or GELSAFE (if it is available a transilluminator). 
> You need:
Micropipettes, PCR, microtubes, and thermal cycler.

icon-refPCR5           25 students

GENETICALLY MODIFIED CORN DETECTION

This kit includes all material you need for the detection of transgenic Bt corn using the PCR technique. The kit is provided with several samples of corn powder and the material to the extraction of DNA from these samples and other possible chosen by the students.

> Kit includes:
Corn powder sample, MIX PCR, normal DNA positive control, transgenic DNA positive control, agarose, TAE and DANABLUE-FlashBlue or GELSAFE (if it is available a transilluminator). 
> You need:
Micropipettes, PCR, microtubes, and thermal cycler.

icon-refPCR6           25 students

EXPLORING THE GENETICS OF TASTE: SNP ANALYSIS OF THE PTC GEN BY PCR

DNA sequences that vary between individuals are known as polymorphisms. Students will prepare their own DNA fron bucal cells that will be used to amplify the TAS2R38 gene, wich is responsible for the ability to detect bitter taste. Subsequently, the purification of the PCR product will be carried out and it will be digested with the restriction enzyme HaeIII to identify the presence or absence of the SNP linked to the phenotype of detecting the bitter taste in PTC papers.

> Kit includes:
MIX PCR, positive control,  agarose, TAE, GELSAFE, Kit purification PCR fragment, restriction enzyme HaeIII and control papers. 
> You need:
Micropipettes, PCR, microtubes, thermal cycler, microcentrifuge and water bath for incubation at 37ºC.

icon-refPCR7           25 students


 Download english protocol 

EXPLORING GENETICS TASTE

INTRODUCTION TO REAL TIME PCR

This kit is an introduction to the Real Time PCR. Students will be able to prepare standard curves and quantify samples problema. The accumulating amplified product can be detected at each cycle with fluorescent dyes.

The presentation are individuals ready-to-use microtubes containing all the components needed to perform the quantification PCR assay.

> Kit includes:
MIX Individual microtubes containing dehydrated mixture of specific primers and labelled probe; DNase/RNase free wáter; Standard Template; Standard Buffer. 
> You need:
Micropipettes, PCR microtubes and Real Time Machine.

icon-refPCR8           25 students
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